KRT7 Antibody / Cytokeratin 7

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NSJ Bioreagents
Product Code: NSJ-V2658SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2658SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: KRT7/760
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).
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IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).
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Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760); Red=isotype control, Blue= recombinant Cytokeratin 7 antibody.
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Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760, green) and Reddot nuclear stain (red).
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IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).
IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).
Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760); Red=isotype control, Blue= recombinant Cytokeratin 7 antibody.
Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760, green) and Reddot nuclear stain (red).

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.
Limitation:
This Cytokeratin 7 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P08729