Keratin 6B Antibody / Cytokeratin 6B

Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT

Optimal dilution of the Cytokeratin 6B antibody should be determined by the researcher.

This MAb recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant full-length human KRT6B protein was used as the immunogen for the Cytokeratin 6B antibody.

This Cytokeratin 6B antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography

P04259