Cytokeratin 20 Antibody / CK20

NSJ Bioreagents
Product Code: NSJ-V8275SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8275SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: KRT20/3145
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone KRT20/3145). Predicted molecular weight ~46 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free Cytokeratin 20 antibody (clone KRT20/3145) as confirmation of integrity and purity.

IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone KRT20/3145). Predicted molecular weight ~46 kDa.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 20 antibody (clone KRT20/3145) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 0.1-0.2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.
Limitation:
This Cytokeratin 20 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P35900