Integrin alpha E Antibody / CD103

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NSJ Bioreagents
Product Code: NSJ-V3837SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3837SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: ITGAE/2474
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the CD103 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human tonsil with CD103 antibody (clone ITGAE/2474). HIER: boil tissue sections in pH9, 1mM EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human colon with CD103 antibody (clone ITGAE/2474). HIER: boil tissue sections in pH9, 1mM EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.
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SDS-PAGE analysis of purified, BSA-free CD103 antibody (clone ITGAE/2474) as confirmation of integrity and purity.
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IHC testing of FFPE human tonsil with CD103 antibody (clone ITGAE/2474). HIER: boil tissue sections in pH9, 1mM EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human colon with CD103 antibody (clone ITGAE/2474). HIER: boil tissue sections in pH9, 1mM EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free CD103 antibody (clone ITGAE/2474) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Titering of the CD103 antibody may be required for optimal performance.
Description:
This antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E-beta-7 integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. This antibody is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 775-855 from the human protein was used as the immunogen for the CD103 antibody.
Limitation:
This CD103 antibody is available for research use only.
Localization:
Cell membrane, cytoplasm
Purity:
Protein G affinity chromatography
Uniprot #:
P38570