IgM Heavy Chain Antibody

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

The optimal dilution of the recombinant IgM antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant heavy chain of human IgM was used as the immunogen for this recombinant IgM antibody.

This recombinant IgM antibody is available for research use only.

Cytoplasm, cell surface, secreted

Protein A affinity chromatography

P01871, P20769