CD20 Antibody

NSJ Bioreagents
Product Code: NSJ-V7238SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7238SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: IGEL/1497R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

1 / 2
IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min.~
2 / 2
Flow cytometry testing of human Raji cells with recombinant CD20 antibody (clone IGEL/1497R)

IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min.~
Flow cytometry testing of human Raji cells with recombinant CD20 antibody (clone IGEL/1497R)

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Titering of the recombinant CD20 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.
Limitation:
This recombinant CD20 antibody is available for research use only.
Localization:
Predominantly cell surface with some cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P11836