CD34 Antibody
| Code | Size | Price |
|---|
| NSJ-V2065PE-100T | 100 Tests | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: ICO-115
Regulatory Status: RUO
Target Species:
- Human
- Rat
Application: Fluorescence-activated cell sorting (FACS)
Storage:
Store the CD34 antibody at 2-8°C. Conjugate is light sensitive store in the dark.
Documents
Further Information
Application Details:
Flow Cytometry: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood),Immunofluorescence: 1:50-1:100
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Description:
This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi?s sarcoma, but shows low specificity.
Format:
PE Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Gene ID #:
947 (Human)
Immunogen:
Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody.
Limitation:
This antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography