CD34 Antibody

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NSJ Bioreagents
Product Code: NSJ-V2065SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2065SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: ICO-115
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (ICO-115, red) and isotype control antibody (green). The PPI-negative cell population was gated for analysis.~
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SDS-PAGE Analysis of Purified, BSA-Free CD34 Antibo
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Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (ICO-115, red) and isotype control antibody (green). The PPI-negative cell population was gated for analysis.~
SDS-PAGE Analysis of Purified, BSA-Free CD34 Antibo

Documents

Further Information

Application Details:
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 0.5-1ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Description:
This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi?s sarcoma, but shows low specificity.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
947
Immunogen:
Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody.
Limitation:
This CD34 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography