ICAM-3 Antibody / CD50

NSJ Bioreagents
Product Code: NSJ-V7507SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7507SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: ICAM3/2873R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Blocking
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant ICAM3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 2
IHC staining of FFPE human tonsil tissue with recombinant ICAM3 antibody (clone ICAM3/2873R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
SDS-PAGE analysis of purified, BSA

IHC staining of FFPE human tonsil tissue with recombinant ICAM3 antibody (clone ICAM3/2873R). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.~
SDS-PAGE analysis of purified, BSA

Documents

Further Information

Application Details:
Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (order BSA/sodium azide-free format),Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant ICAM3 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length recombinant human protein was used as the immunogen for the recombinant ICAM3 antibody.
Limitation:
This recombinant ICAM3 antibody is available for research use only.
Localization:
Membrane, cytoplasm
Purity:
Protein A affinity chromatography
Uniprot #:
P32942