HSP60 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3575SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3575SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: HSPD1/2206R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of human HeLa lysate with recombinant HSP60 antibody (clone HSPD1/2206R). Predicted molecular weight: ~60 kDa.
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IHC testing of FFPE dog pancreas tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human HeLa lysate with recombinant HSP60 antibody (clone HSPD1/2206R). Predicted molecular weight: ~60 kDa.
IHC testing of FFPE dog pancreas tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
HSP60 has been implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant HSP60 antibody.
Limitation:
This recombinant HSP60 antibody is available for research use only.
Localization:
Cytoplasm (mitochondria)
Purity:
Protein A affinity chromatography
Uniprot #:
P10809