HER2 Antibody / ErbB2

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NSJ Bioreagents
Product Code: NSJ-V2108SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2108SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: HRB2/451
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Immunofluorescent staining of PFA-fixed human MCF-7 cells with HER2 antibody (green) and Reddot nuclear stain (red).
2 / 5
Flow cytometry testing of human MCF-7 cells with HER2 antibody at 1ug/10^6 cells; Red=isotype control, Blue= HER2 antibody.
3 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 antibody (clone HRB2/451). These results demonstrate the foremost specificity of the HRB2/451 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 5
Flow cytometry testing of human SK-BR3 cells with HER2 antibody at 1ug/million cells; Red=isotype control, Blue= HER2 antibody.
5 / 5
Immunofluorescent staining of MeOH-fixed human MCF-7 cells with HER2 antibody (green) and Reddot nuclear stain (red).
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Immunofluorescent staining of PFA-fixed human MCF-7 cells with HER2 antibody (green) and Reddot nuclear stain (red).
Flow cytometry testing of human MCF-7 cells with HER2 antibody at 1ug/10^6 cells; Red=isotype control, Blue= HER2 antibody.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 antibody (clone HRB2/451). These results demonstrate the foremost specificity of the HRB2/451 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Flow cytometry testing of human SK-BR3 cells with HER2 antibody at 1ug/million cells; Red=isotype control, Blue= HER2 antibody.
Immunofluorescent staining of MeOH-fixed human MCF-7 cells with HER2 antibody (green) and Reddot nuclear stain (red).

Documents

Further Information

Application Details:
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.
Description:
This mAb is specific to HER2 / ErbB2 and shows minimal cross-reaction with other members of the family. HER2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
2064
Immunogen:
Recombinant human protein was used as the immunogen for this HER2 antibody.
Limitation:
This HER2 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography