CD34 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7384SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7384SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: HPCA1/2598R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human renal cell carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human colon carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human tonsil with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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SDS-PAGE analysis of purified, BSA-free recombinant CD34 antibody (clone HPCA1/2598R) as confirmation of integrity and purity.
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Flow cytometry testing of human Jurkat cells with CD34 antibody (clone HPCA1/2598R); Red=isotype control, Blue= CD34 antibody.
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IHC testing of FFPE human renal cell carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human colon carcinoma with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human tonsil with recombinant CD34 antibody (clone HPCA1/2598R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant CD34 antibody (clone HPCA1/2598R) as confirmation of integrity and purity.
Flow cytometry testing of human Jurkat cells with CD34 antibody (clone HPCA1/2598R); Red=isotype control, Blue= CD34 antibody.

Documents

Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant CD34 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.
Limitation:
This recombinant CD34 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P28906