Kappa Light Chain Antibody Cocktail

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

The optimal dilution of the Kappa Light Chain antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Purified human Ig kappa chain (HP6053) and human B-lymphoma cells (L1C1) were used as the immunogens for this Kappa Light Chain antibody cocktail.

This Kappa Light Chain antibody cocktail is available for research use only.

Cell Surface, cytoplasmic and secreted

Protein G affinity chromatography

P01601, P01834