Histone H1 Antibody

NSJ Bioreagents
Product Code: NSJ-V7278SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7278SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: HH1/1784R
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
IHC testing of FFPE human pancreas and recombinant Histone H1 antibody (clone HH1/1784R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
2 / 3
Immunofluorescent staining of permeabilized human HeLa cells with recombinant Histone H1 antibody (green, clone HH1/1784R) and Phalloidin.
3 / 3
Western blot testing of human heart lysate with recombinant Histone H1 (clone HH1/1784R). Observed molecular weight ~22/30-33 kDa (unmodified/modified).

IHC testing of FFPE human pancreas and recombinant Histone H1 antibody (clone HH1/1784R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Immunofluorescent staining of permeabilized human HeLa cells with recombinant Histone H1 antibody (green, clone HH1/1784R) and Phalloidin.
Western blot testing of human heart lysate with recombinant Histone H1 (clone HH1/1784R). Observed molecular weight ~22/30-33 kDa (unmodified/modified).

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the rabbit monoclonal recombinant Histone H1 antibody.
Limitation:
This recombinant Histone H1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A affinity chromatography
Uniprot #:
P07305