HCG-beta Antibody Cocktail

Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

The optimal dilution of the HCG-beta antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This mAb reacts with a protein of 22kDa, identified as the beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG mAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant hCG beta protein was used as the immunogens for this hCG beta antibody cocktail.

This HCG-beta antibody cocktail is available for research use only.

Cytoplasmic, Secreted

Protein G affinity chromatography

P01233