hCG alpha Antibody / Luteinizing Hormone alpha

NSJ Bioreagents
Product Code: NSJ-V2091SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2091SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: HCGa/53
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the hCG alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of human placenta stained with hCG alpha antibody (HCGa/53). Note specific membrane staining.~
2 / 2
SDS-PAGE analysis of purified, BSA-free hCG alpha antibody (clone HCGa/53) as confirmation of integrity and purity.

IHC testing of human placenta stained with hCG alpha antibody (HCGa/53). Note specific membrane staining.~
SDS-PAGE analysis of purified, BSA-free hCG alpha antibody (clone HCGa/53) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (HIER is not recommended for this antibody)
Application Note:
Variations in protocols, secondaries and substrates may require the hCG alpha antibody to be titered for optimal performance.

1. No pretreatment required for FFPE section testing. HIER is not recommended.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant protein was used as the immunogen for this hCG alpha antibody.
Limitation:
This hCG alpha antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Protein G purified monoclonal antibody
Uniprot #:
P01215