EGF Receptor / EGFR Antibody

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NSJ Bioreagents
Product Code: NSJ-V2105CF488
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2105CF488-100T100 Tests£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: GFR450
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the EGFR antibody at 2-8oC protected from light.

Images

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Left: Immunofluorescent staining of human A431 cells with CF488-conjugated EGFR antibody (clone GFR450, green) and DAPI nuclear stain (blue). Right: isotype control.
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Flow cytometry testing of human A431 cells with EGFR antibody (clone GFR450); Black=cells alone, Gray=isotype control, Green= EGFR antibody.
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Flow cytometry testing of mouse NIH3T3 cells with EGFR antibody (clone GFR450); Black=cells alone, Gray=isotype control, Green= EGFR antibody. This antibody is not suitable for mouse sample testing.
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Left: Immunofluorescent staining of human A431 cells with CF488-conjugated EGFR antibody (clone GFR450, green) and DAPI nuclear stain (blue). Right: isotype control.
Flow cytometry testing of human A431 cells with EGFR antibody (clone GFR450); Black=cells alone, Gray=isotype control, Green= EGFR antibody.
Flow cytometry testing of mouse NIH3T3 cells with EGFR antibody (clone GFR450); Black=cells alone, Gray=isotype control, Green= EGFR antibody. This antibody is not suitable for mouse sample testing.

Documents

Further Information

Application Details:
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100
Application Note:
Optimal dilution of the EGFR antibody should be determined by the researcher.
Description:
This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Format:
CF488 Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant extracellular domain of human EGFR protein was used as the immunogen for the EGFR antibody.
Limitation:
This EGFR antibody is available for research use only.
Purity:
Protein G affinity chromatography
Uniprot #:
P00533