EGF Receptor / EGFRvIII Antibody

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NSJ Bioreagents
Product Code: NSJ-V3980SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3980SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: GFR/2600R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant EGFRvIII antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human lung squamous cell carcinoma with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.~
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IHC te
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IHC testing of FFPE human lung squamous cell carcinoma with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.~
IHC te

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
Application Note:
Titering of the recombinant EGFRvIII antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids LEEKKGNYVVTDHC were used as the immunogen for the recombinant EGFRvIII antibody. This is the predicted sequence of the exon 2-7 delete fusion region.
Limitation:
This recombinant EGFRvIII antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Uniprot #:
P00533