GFAP Antibody / Glial Fibrillary Acidic Protein

NSJ Bioreagents
Product Code: NSJ-V3865SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3865SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: GFAP/2076
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Glial Fibrillary Acidic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Flow cytometry testing of fixed human T98G cells with Glial Fibrillary Acidic Protein antibody (clone GFAP/2076); Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.~
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IHC testing of FFPE human cerebellum stained with Gli

Flow cytometry testing of fixed human T98G cells with Glial Fibrillary Acidic Protein antibody (clone GFAP/2076); Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.~
IHC testing of FFPE human cerebellum stained with Gli

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.
Description:
This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 101-200 were used as the immunogen for the Glial Fibrillary Acidic Protein antibody.
Limitation:
This Glial Fibrillary Acidic Protein antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P14136