FTL Antibody / Ferritin light chain

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NSJ Bioreagents
Product Code: NSJ-V3352SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3352SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: FTL/1388
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Ferritin Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1388). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1388). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
3 / 4
Western blot testing of human kidney lysate with Ferritin Light Chain antibody. Predicted molecular weight: ~20 kDa.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Ferritin Light Chain antibody. These results demonstrate the foremost specificity of the FTL/1388 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1388). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1388). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Western blot testing of human kidney lysate with Ferritin Light Chain antibody. Predicted molecular weight: ~20 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Ferritin Light Chain antibody. These results demonstrate the foremost specificity of the FTL/1388 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Western blot 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Ferritin Light Chain antibody should be determined by the researcher.
Description:
The FTL gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. This gene has multiple pseudogenes.

Although ferritin light chain has no ferroxidase activity, the light chain may be responsible for the electron transfer across the ferritin protein cage. [Wiki]

Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin Light Chain antibody.
Limitation:
This Ferritin Light Chain antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P02792