FOXA1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3292SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3292SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: FOXA1/1512
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the FOXA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
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IHC testing of FFPE human prostate with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
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Immunofluorescent staining of PFA-fixed human MCF7 cells with FOXA1 antibody (clone FOXA1/1512, green) and Phalloiden (red).
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FACS testing of PFA-fixed human MCF-7 cells with FOXA1 antibody (clone FOXA1/1512); Red=isotype control, Blue= FOXA1 antibody.
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SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1512). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
IHC testing of FFPE human prostate with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
Immunofluorescent staining of PFA-fixed human MCF7 cells with FOXA1 antibody (clone FOXA1/1512, green) and Phalloiden (red).
FACS testing of PFA-fixed human MCF-7 cells with FOXA1 antibody (clone FOXA1/1512); Red=isotype control, Blue= FOXA1 antibody.
SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1512). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the FOXA1 antibody should be determined by the researcher.
Description:
FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin as a pioneer factor. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

FOXA1 in breast cancer is highly correlated with ERa+, GATA3+, and PR+ protein expression as well as endocrine signaling. FOXA1 acts as a pioneer factor for ERa in ERa+ breast cancer, and its expression might identify ERa+ cancers that undergo rapid reprogramming of ERa signaling that is associated with poor outcomes and treatment resistance. Conversely, in ERa+/- breast cancer FOXA1 is highly correlated with low-grade morphology and improved disease free survival. FOXA1 is a downstream target of GATA3 in the mammary gland. Expression in ERa+/- cancers may identify a subset of tumors that is responsive to other endocrine therapies such as androgen receptor antagonist treatment. [Wiki]

Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
3169
Immunogen:
A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
Limitation:
This FOXA1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P55317