HER2 Antibody / ErbB2 / Extracellular region

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NSJ Bioreagents
Product Code: NSJ-V8633SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8633SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit
Antibody Clonality: Monoclonal
Antibody Clone: ERBB2/4439
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/4439). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/4439). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ERBB2/4439, green) and Phalloidin (red).
4 / 5
Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 antibody (clone ERBB2/4439); Red=isotype control, Blue= HER2 antibody.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 antibody. These results demonstrate the foremost specificity of the ERBB2/4439 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/4439). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/4439). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ERBB2/4439, green) and Phalloidin (red).
Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 antibody (clone ERBB2/4439); Red=isotype control, Blue= HER2 antibody.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 antibody. These results demonstrate the foremost specificity of the ERBB2/4439 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the HER2 antibody should be determined by the researcher.
Description:
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant protein encoding the extracellular domain of human c-erbB2 was used as the immunogen for the HER2 antibody.
Limitation:
This HER2 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Uniprot #:
IDP04626