HER2 Antibody / ErbB2
| Code | Size | Price |
|---|
| NSJ-V3839SAF-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: ERBB2/2452
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER2 antibody to be titered up or down for optimal performance.
Description:
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 311-462 from the human protein was used as the immunogen for this HER2 antibody.
Limitation:
This HER2 antibody is available for research use only.
Localization:
Cell membrane (This mAb binds to the extracellular/cell surface region of the protein)
Purity:
Protein G affinity chromatography
Uniprot #:
P04626