Estrogen Receptor beta Antibody

Flow Cytometry: 5ul/test/million cells or 5ul/test/100ul of whole blood,Immunofluorescence: 1:50-1:100 for 30 minutes at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including alpha and beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER alpha and beta are differentially activated by various ligands. Ligand interaction triggers a cascade of events, including dissociation from heat shock proteins, recepter dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and beta may be regulated by distinct mechanisms even though they share many functional characteristics.

PE Conjugate

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

2100 (Human)

The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.

This Estrogen Receptor beta antibody is available for research use only.

Predominantly nuclear

Protein G affinity chromatography

Q92731