HER2 Antibody / ErbB2
| Code | Size | Price |
|---|
| NSJ-V2377SAF-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: ERB2/776
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
Storage:
Store the HER-2 antibody at 2-8°C.
Documents
Further Information
Application Details:
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER-2 antibody to be titered up or down for optimal performance.
1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
2. This HER2 antibody is specific to the intracellular region of the protein.
1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
2. This HER2 antibody is specific to the intracellular region of the protein.
Description:
ErbB2, also called HER2, is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. This ErbB2 antibody shows minimal cross-reaction with other members of the family. The protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
2064
Immunogen:
Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)
Limitation:
This HER-2 antibody is available for research use only.
Localization:
Extracellular/Intracellular cell membrane (this mAb is binds to the intracellular portion of the protein)
Purity:
Protein G affinity chromatography