Estrogen Receptor alpha Antibody

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NSJ Bioreagents
Product Code: NSJ-V2113SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2113SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: ER506
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the ER antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human endometrium with ER antibody (clone ER506).
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Intracellular FACS testing of human MCF-7 cells: Black=cells alone; Green=isotype control; Red= <a href=../tds/estrogen-receptor-antibody-pe-conjugate-er506-v2113pe>PE conjugated ER antibody (V2113PE)</a>
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Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~67 kDa.
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SDS-PAGE analysis of purified, BSA-free ER antibody (clone ER506) as confirmation of integrity and purity.
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IHC testing of FFPE human endometrium with ER antibody (clone ER506).
Intracellular FACS testing of human MCF-7 cells: Black=cells alone; Green=isotype control; Red= <a href=../tds/estrogen-receptor-antibody-pe-conjugate-er506-v2113pe>PE conjugated ER antibody (V2113PE)</a>
Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~67 kDa.
SDS-PAGE analysis of purified, BSA-free ER antibody (clone ER506) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml (1)
Application Note:
Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.

1. FFPE testing requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and cooling at RT for 20 minutes.

Description:
Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Clone ER506 antibody strongly stains nuclei of epithelial cells in breast carcinomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
2099
Immunogen:
Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
Limitation:
This ER antibody is available for research use only.
Localization:
Intracellular, Nuclear
Purity:
Protein G affinity chromatography