EMI1 Antibody (Early Mitotic Inhibitor-1)

Western blot: 1-2ug/ml for 60 min at RT,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the EMI1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.

This EMI1 antibody is available for research use only.

Nuclear

Protein G affinity chromatography

Q9UKT4