EpCAM Antibody

NSJ Bioreagents
Product Code: NSJ-V2962PE
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2962PE-100T100 Tests£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: EGP40/837
Regulatory Status: RUO
Target Species: Human
Application: Fluorescence-activated cell sorting (FACS)
Storage:
Store the EpCAM antibody at 2-8oC protected from light.

Images

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Flow cytometry testing of human MCF-7 cells with EpCAM antibody; Black=cells alone, Green=isotype control, Red= EpCAM antibody.

Flow cytometry testing of human MCF-7 cells with EpCAM antibody; Black=cells alone, Green=isotype control, Red= EpCAM antibody.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells
Application Note:
Optimal dilution of the CD7 antibody should be determined by the researcher.
Description:
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format:
PE Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the EpCAM antibody.
Limitation:
This EpCAM antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P16422