EpCAM Antibody / Extracellular domain

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NSJ Bioreagents
Product Code: NSJ-V3559SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3559SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: EGP40/2041R
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Feline (cat)
  • Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human thyroid carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2 / 5
IHC testing of FFPE human basal cell carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 5
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2041R) as confirmation of integrity and purity.
4 / 5
IHC testing of FFPE dog bladder and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.
5 / 5
IHC testing of FFPE cat small intestine and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.
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IHC testing of FFPE human thyroid carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human basal cell carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2041R) as confirmation of integrity and purity.
IHC testing of FFPE dog bladder and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.
IHC testing of FFPE cat small intestine and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.

Documents

Further Information

Application Details:
Western blot:1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Titering of the recombinant EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas; in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.
Limitation:
This recombinant EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P16422