DOG1 Antibody / TMEM16A / ANO1

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

The optimal dilution of the DOG-1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Amino acids 904-986 from the human protein were used as the immunogen for this DOG-1 antibody.

This DOG-1 antibody is available for research use only.

Cell Surface and Cytoplasm

Protein G affinity chromatography

Q5XXA6