DOG1 Antibody / TMEM16A / ANO1

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NSJ Bioreagents
Product Code: NSJ-V3473IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3473IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: DG1/1486
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the DOG1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (clone DG1/1486). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.~
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SDS-PAGE analysis of purified, BSA-free DOG 1 antibo
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IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (clone DG1/1486). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.~
SDS-PAGE analysis of purified, BSA-free DOG 1 antibo

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.
Description:
Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST s), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GIST s. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94.4% vs. 94.7%.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.
Limitation:
This DOG1 antibody is available for research use only.
Localization:
Cell Surface and Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
Q5XXA6