Cyclin D1 Antibody / CCND1

Contact us
NSJ Bioreagents
Product Code: NSJ-V2006SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2006SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: DCS-6
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cyclin D1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear staining of tumor cells is seen.
2 / 5
Western blot testing of human and mouse samples with DCS-6 mAb. Predicted molecular weight: 32-36 kDa.
3 / 5
FACS testing of Jurkat cells: Black=cells alone; Green=isotype control; Red=<a href=../cyclin-d1-antibody-pe-conjugate-dcs-6-v2006pe>Cyclin D1 antibody PE conjugate (V2006PE)</a>
4 / 5
FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=<a href=../cyclin-d1-antibody-pe-conjugate-dcs-6-v2006pe>Cyclin D1 antibody PE conjugate (V2006PE)</a>
5 / 5
SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (DCS-6). Confirmation of Integrity and Purity of the Antibody.
❮❯

IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear staining of tumor cells is seen.
Western blot testing of human and mouse samples with DCS-6 mAb. Predicted molecular weight: 32-36 kDa.
FACS testing of Jurkat cells: Black=cells alone; Green=isotype control; Red=<a href=../cyclin-d1-antibody-pe-conjugate-dcs-6-v2006pe>Cyclin D1 antibody PE conjugate (V2006PE)</a>
FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=<a href=../cyclin-d1-antibody-pe-conjugate-dcs-6-v2006pe>Cyclin D1 antibody PE conjugate (V2006PE)</a>
SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (DCS-6). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a protein of 36kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
595
Immunogen:
Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.
Limitation:
This Cyclin D1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography