Cytokeratin 18 Antibody

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NSJ Bioreagents
Product Code: NSJ-V2178SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2178SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: DA7
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human colon cancer stained with Cytokeratin 18 antibody (clone DA7).
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Western blot testing of human HeLa cell lysate using Cytokeratin 18 antibody (clone DA7). Predicted molecular weight ~48 kDa.
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Western blot testing of human HCT116 cell lysate using Cytokeratin 18 antibody (clone DA7). Predicted molecular weight ~48 kDa.
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SDS-PAGE analysis of purified, BSA-free Cytokeratin 18 antibody (clone DA7) as confirmation of integrity and purity.
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IHC testing of FFPE human colon cancer stained with Cytokeratin 18 antibody (clone DA7).
Western blot testing of human HeLa cell lysate using Cytokeratin 18 antibody (clone DA7). Predicted molecular weight ~48 kDa.
Western blot testing of human HCT116 cell lysate using Cytokeratin 18 antibody (clone DA7). Predicted molecular weight ~48 kDa.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 18 antibody (clone DA7) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This cytokeratin 18 antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 18 and 8 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
3875
Immunogen:
Human breast cancer PMC 42 cells were used as the immunogen for this antibody.
Limitation:
This Cytokeratin 18 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography