KRT8 Antibody / Cytokeratin 8
Code | Size | Price |
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NSJ-V7042SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: CYKN8-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store the Cytokeratin 8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
3856
Immunogen:
Recombinant human protein was used as the immunogen for this Cytokeratin 8 antibody.
Limitation:
This Cytokeratin 8 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography