CTSD Antibody / Cathepsin D

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NSJ Bioreagents
Product Code: NSJ-V7737SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7737SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: CTSD/3276
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cathepsin D antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Immunofluorescent staining of permeabilized human MCF7 cells with Cathepsin D antibody (clone CTSD/3276, green) and Reddot nuclear stain (red).
2 / 5
IHC staining of FFPE human liver with Cathepsin D antibody (clone CTSD/3276). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 5
Western blot testing of human liver lysate with Cathepsin D antibody.
4 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Cathepsin D antibody (clone CTSD/3276). These results demonstrate the foremost specificity of the CTSD/3276 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
5 / 5
SDS-PAGE analysis of purified, BSA-free Cathepsin D antibody (clone CTSD/3276) as confirmation of integrity and purity.
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Immunofluorescent staining of permeabilized human MCF7 cells with Cathepsin D antibody (clone CTSD/3276, green) and Reddot nuclear stain (red).
IHC staining of FFPE human liver with Cathepsin D antibody (clone CTSD/3276). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human liver lysate with Cathepsin D antibody.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Cathepsin D antibody (clone CTSD/3276). These results demonstrate the foremost specificity of the CTSD/3276 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Cathepsin D antibody (clone CTSD/3276) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins. It is synthesized as an inactive 52kDa preprocathepsin D that is cleaved and glycosylated to form a 48kDa procathepsin D and then further cleaved to produce 34kDa and 14kDa subunits (heavy and light chains, respectively). Cathepsin D exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human partial protein (amino acids 104-250) was used as the immunogen of the Cathepsin D antibody.
Limitation:
This Cathepsin D antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P07339