Multi Cytokeratin Antibody
Code | Size | Price |
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NSJ-V3718SAF-100UG | 100 ug | £534.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CTKNM-1R
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details:
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.
1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human epithelial cell keratin was used as the immunogen for the recombinant Multi Cytokeratin antibody.
Limitation:
This recombinant Multi Cytokeratin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography