KRT16 Antibody / Cytokeratin 16

NSJ Bioreagents
Product Code: NSJ-V3737SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3737SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CTKN16-1R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Cytokeratin 16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 1
IHC testing of FFPE human skin with recombinant Cytokeratin 16 antibody (clone CTKN16-1R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human skin with recombinant Cytokeratin 16 antibody (clone CTKN16-1R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 16 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Cytokeratin 16 is an epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A C-terminal region peptide was used as the immunogen for the recombinant Cytokeratin 16 antibody.
Limitation:
This recombinant Cytokeratin 16 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P08779