KRT15 Antibody / Cytokeratin 15

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NSJ Bioreagents
Product Code: NSJ-V8127SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8127SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c kappa
Antibody Clonality: Monoclonal
Antibody Clone: CTKN15-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Western blot testing of human thymus tissue and HCT-116 cell lysate with CK15 antibody (clone CTKN15-1). Expected molecular weight ~50 kDa.
2 / 3
Immunofluorescent staining of MeOH-fixed human MCF7 cells with Cytokeratin 15 antibody (clone CTKN15-1, green) and Reddot nuclear stain (red).
3 / 3
IHC staining of FFPE human prostate carcinoma with Cytokeratin 15 antibody (clone CTKN15-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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Western blot testing of human thymus tissue and HCT-116 cell lysate with CK15 antibody (clone CTKN15-1). Expected molecular weight ~50 kDa.
Immunofluorescent staining of MeOH-fixed human MCF7 cells with Cytokeratin 15 antibody (clone CTKN15-1, green) and Reddot nuclear stain (red).
IHC staining of FFPE human prostate carcinoma with Cytokeratin 15 antibody (clone CTKN15-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Cytokeratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the Cytokeratin 15 antibody.
Limitation:
This Cytokeratin 15 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P19012