CD5 Antibody

Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Functional studies (order BSA/sodium azide-free format),Immunoprecipitation (0.5-1ug/500ug protein lysate)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD5 antibody to be titered up or down for optimal performance.

This antibody recognizes a 67kDa transmembrane protein, which is identified as CD5. It is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in Tcell areas. CD5 antibody is a pan Tcell marker that also reacts with a range of neoplastic Bcells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large Bcell lymphoma. Aberrant expression is useful in making a diagnosis of mature Tcell neoplasms. CD5 antibody detection is diagnostic in CLL/SLL within a panel of other Bcell markers, especially one that includes anti-CD23. CD5 antibody is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, it can be used in distinguishing thymic carcinoma (+) from thymoma (-). It does not react with granulocytes or monocytes.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

921

Stimulated human leukocytes were used as the immunogen for this CD5 antibody.

This CD5 antibody is available for research use only.

Cell surface

Protein G affinity chromatography