Golgi Antibody

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NSJ Bioreagents
Product Code: NSJ-V7095IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7095IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: CRFG1
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human pancreas and Golgi antibody (clone CRFG1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA followed by cooling at RT for 20 min., prior to staining.
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IHC testing of FFPE human pancreas and Golgi antibody (clone CRFG1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA followed by cooling at RT for 20 min., prior to staining.

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Titering of the Golgi antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Part of the cellular endomembrane system, the Golgi apparatus packages proteins into membrane-bound vesicles inside the cell before the vesicles are sent to their destination. The Golgi apparatus resides at the intersection of the secretory, lysosomal, and endocytic pathways. It is of particular importance in processing proteins for secretion, containing a set of glycosylation enzymes that attach various sugar monomers to proteins as the proteins move through the apparatus. [Wiki]
This antibody is useful in staining the Golgi apparatus in cell or tissue preparations and subcellular fractions.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
The Golgi fraction from human liver cells was used as the immunogen for the Golgi antibody. Not suitable for mouse and rat sample testing.
Limitation:
This Golgi antibody is available for research use only.
Localization:
Golgi complex in cytoplasm
Purity:
Protein G affinity chromatography
Uniprot #:
Q92896