SERPINB3 Antibody / SCCA-1

Western blot: 0.5-1ug/ml

Optimal dilution of the SERPINB3 antibody should be determined by the researcher.

Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine proteases, such as neutrophil elastase. Serpins are family of serine protease inhibitors, contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Serine proteases bind to this substrate mimic in a 1:1 stoichiometric fashion and become catalytically inactive. Aberrant expression of serpin family members can contribute to a number of conditions, including emphysema (-1 antitrypsin deficiency), fatal bleeding (elastase to Thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of 'squamous cell carcinoma antigen', known as SCCA1 and SERPINB3, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers). SCCA1/SERPINB3 expression has been demonstrated to promote oncogenic transformation and epithelial-mesenchymal transition (EMT) in mammary epithelial cells, and its silencing in breast cancer cells has been shown to halt their proliferation.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant human protein was used as the immunogen for this SERPINB3 antibody.

This SERPINB3 antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography

P29508