PSMD4 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8472SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8472SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: CPTC-PSMD4-3
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store the PSMD4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Western blot testing of human liver lysate with PSMD4 antibody. Predicted molecular weight ~41 kDa, routinely observed at ~50 kDa.
2 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PSMD4 antibody. These results demonstrate the foremost specificity of the CPTC-PSMD4-3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
3 / 3
SDS-PAGE analysis of purified, BSA-free PSMD4 antibody as confirmation of integrity and purity.
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Western blot testing of human liver lysate with PSMD4 antibody. Predicted molecular weight ~41 kDa, routinely observed at ~50 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PSMD4 antibody. These results demonstrate the foremost specificity of the CPTC-PSMD4-3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free PSMD4 antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the PSMD4 antibody should be determined by the researcher.
Description:
In eukaryotic cells, selective breakdown of cellular proteins is ensured by two distinct pathways. First, appropriate proteins are tagged for degradation by ubiquitination. Second, these multiubiquitinated proteins are degraded by the highly selective 26S Proteasome protein-destroying machinery. At specific stages of development, embryo- and tissue-specific components of the 26S Proteasome are formed, which are termed Rpn10a through Rpn10e, or alternatively pUb-R2 through pUb-R5. All members of this family can be generated by a single Rpn10 gene by developmentally regulated alternative splicing. The pUb-R2 subunit, originally identified as S5a (also designated antisecretory factor and multiubiquitin chain binding protein) is ubiquitously expressed and may perform proteolysis constitutively in a wide variety of cells. pUb-R4 and pUb-R5 may have embryo- or tissue-specific expression and may play specialized roles in early embryonic development.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human PSMD4 protein was used as the immunogen for the PSMD4 antibody.
Limitation:
This PSMD4 antibody is available for research use only.
Localization:
Nucleus and cytoplasm
Purity:
Protein G affinity chromatography
Uniprot #:
P55036