HSP27 Antibody / HSPB1

Contact us
NSJ Bioreagents
Product Code: NSJ-V7422SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7422SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: CPTC-HSPB1-2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC staining of FFPE human prostate tissue with HSP27 antibody (clone CPTC-HSPB1-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 4
Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone CPTC-HSPB1-2, green) and Reddot nuclear stain (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone CPTC-HSPB1-2) as confirmation of integrity and purity.
4 / 4
Western blot testing of human liver tissue lysate with HSP27 antibody (clone CPTC-HSPB1-2). Predicted molecular weight: 23-27 kDa.
❮❯

IHC staining of FFPE human prostate tissue with HSP27 antibody (clone CPTC-HSPB1-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone CPTC-HSPB1-2, green) and Reddot nuclear stain (red).
SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone CPTC-HSPB1-2) as confirmation of integrity and purity.
Western blot testing of human liver tissue lysate with HSP27 antibody (clone CPTC-HSPB1-2). Predicted molecular weight: 23-27 kDa.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the HSP27 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length recombinant human protein was used as the immunogen for the HSP27 antibody.
Limitation:
This HSP27 antibody is available for research use only.
Localization:
Predominantly cytoplasmic with some nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P04792