GLO1 Antibody / Glyoxalase I

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NSJ Bioreagents
Product Code: NSJ-V7812SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7812SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: CPTC-GLO1-3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Glyoxalase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC testing of FFPE human thyroid carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2 / 4
IHC testing of FFPE human prostate carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 4
Western blot testing of human HeLa and HepG2 cell lysate with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Predicted molecular weight ~21 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free Glyoxalase 1 antibody (clone CPTC-GLO1-3) as confirmation of integrity and purity.
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IHC testing of FFPE human thyroid carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human prostate carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human HeLa and HepG2 cell lysate with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Predicted molecular weight ~21 kDa.
SDS-PAGE analysis of purified, BSA-free Glyoxalase 1 antibody (clone CPTC-GLO1-3) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
GLO1 is an enzyme involved in the detoxification of methylgyoxal, a byproduct of glycolysis. GLO1 expression has been demonstrated by several studies to be upregulated in various human malignant tumors, including metastatic melanoma and lung carcinoma, and thus is a target for pharmaceutical development.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length human recombinant protein was used as the immunogen for the Glyoxalase 1 antibody.
Limitation:
This Glyoxalase 1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
Q04760