NSJ Bioreagents

EBAG9 Antibody / Estrogen Receptor Binding Site Associated Antigen 9

Product Code:
 
NSJ-V8199SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CPTC-EBAG9-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the EBAG9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Immunofluorescent staining of permeabilized human MCF7 cells with EBAG9 antibody (green) and Reddot nuclear stain (red).
2 / 3
IHC staining of FFPE human tonsil tissue with EBAG9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free EBAG9 antibody as confirmation of integrity and purity.
❮❯

Immunofluorescent staining of permeabilized human MCF7 cells with EBAG9 antibody (green) and Reddot nuclear stain (red).
IHC staining of FFPE human tonsil tissue with EBAG9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free EBAG9 antibody as confirmation of integrity and purity.

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NSJ-V8199SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
EBAG9, also known as RCAS1, is an estrogen-transcribed protein. Soluble and membranous RCAS1 proteins may play a role in the immune escape of tumor cells by promoting T lymphocyte inhibition of growth and apoptosis. RCAS1 is expressed in a wide variety of cancers, including uterine, ovarian, and lung cancer cells, and acts as a ligand for a putative receptor present on peripheral lymphocytes. RCAS1 is highly expressed not only in cancer cells but also in non-tumor bile duct cells subject to immune attack. RCAS1 inhibits the in vitro growth of receptor-expressing cells and induces apoptosis, contributing to the ability of tumor cells to evade host immune surveillance. High expression of RCAS1 significantly correlates with tumor progression and with poor outcome for many cancer patients.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human full-length EBAG9 protein was used as the immunogen for this EBAG9 antibody.
Limitation:
This EBAG9 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
O00559

Documents