Carboxypeptidase A1 Antibody / CPA1

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NSJ Bioreagents
Product Code: NSJ-V7690SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7690SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: CPA1/2713
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Carboxypeptidase A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC staining of FFPE human pancreas with Carboxypeptidase A1 antibody (clone CPA1/2713). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 4
Western blot testing of human pancreas lysate with Carboxypeptidase A1 antibody (clone CPA1/2713). Predicted molecular weight ~47 kDa.
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Carboxypeptidase A1 antibody (clone CPA1/2713). These results demonstrate the foremost specificity of the CPA1/2713 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free Carboxypeptidase A1 antibody (clone CPA1/2713) as confirmation of integrity and purity.
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IHC staining of FFPE human pancreas with Carboxypeptidase A1 antibody (clone CPA1/2713). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human pancreas lysate with Carboxypeptidase A1 antibody (clone CPA1/2713). Predicted molecular weight ~47 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Carboxypeptidase A1 antibody (clone CPA1/2713). These results demonstrate the foremost specificity of the CPA1/2713 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Carboxypeptidase A1 antibody (clone CPA1/2713) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Human pancreatic procarboxypeptidase A exists as three different active forms, two of which are designated carboxypeptidase A1 (CPA1) and carboxypeptidase A2 (CPA2). CPA1, also known as CPA, is a 419 amino acid secreted monomeric protein that is highly expressed in pancreatic tissue. Functioning as a pancreatic exopeptidase, CPA1 uses zinc as a cofactor to catalyze the release of C-terminal amino acids from a variety of proteins, thereby playing a key role in protein digestion and degradation. Via its catalytic activity, CPA1 is also thought to be involved in zymogen (proenzyme) inhibition, probably functioning to block enzyme activation pathways. Abnormal levels of CPA1 are associated with pancreatic cancer, suggesting a possible role in either tumor progression or tumor suppression events.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length recombinant human protein was used as the immunogen for the Carboxypeptidase A1 antibody.
Limitation:
This Carboxypeptidase A1 antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Protein G affinity chromatography
Uniprot #:
P15085