CD8A Antibody

NSJ Bioreagents
Product Code: NSJ-V3684SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3684SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG k
Antibody Clonality: Recombinant Antibody
Antibody Clone: CDLA8a-2R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant CD8 antibody (with azide) at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

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IHC testing of FFPE human tonsil with recombinant CD8 antibody (clone CDLA8a-2R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human tonsil with recombinant CD8 antibody (clone CDLA8a-2R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD8 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. [Wiki]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human CD8a protein was used as immunogen for this recombinant CD8 antibody.
Limitation:
This recombinant CD8 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Uniprot #:
P01732