CD68 Antibody

NSJ Bioreagents
Product Code: NSJ-V3996SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3996SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CDLA68-3R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human spleen lysate with CD68 antibody (clone CDLA68-3R). Expected molecular weight: 37-110 kDa depending on glycosylation level.~
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IHC staining of FFPE human tonsil with recombinant CD68 antibody (clone CDLA68-3R)

Western blot testing of human spleen lysate with CD68 antibody (clone CDLA68-3R). Expected molecular weight: 37-110 kDa depending on glycosylation level.~
IHC staining of FFPE human tonsil with recombinant CD68 antibody (clone CDLA68-3R)

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant CD68 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full length recombinant human protein was used as the immunogen for the recombinant CD68 antibody.
Limitation:
This recombinant CD68 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein A affinity chromatography
Uniprot #:
P34810