NGF Receptor Antibody / p75NTR / CD271

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

The stated application concentrations are suggested starting points. Titration of the recombinant NGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

CD271/NGFR pays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A portion of amino acids 281-421 from the human protein was used as the immunogen for the recombinant NGFR antibody.

This recombinant NGFR antibody is available for research use only.

Cell surface and cytoplasmic

Protein A affinity chromatography

P08138