CD20 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7096IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7096IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDLA20-1
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

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IHC staining of FFPE human tonsil tissue with CD20 antibody (clone CDLA20-1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.~
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IHC staining of FFPE human lymphoma tissue with CD20 a
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IHC staining of FFPE human tonsil tissue with CD20 antibody (clone CDLA20-1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.~
IHC staining of FFPE human lymphoma tissue with CD20 a

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Titering of the CD20 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant CD20 protein was used as the immunogen for this CD20 antibody. The epitope is known to be extracellular.
Limitation:
This CD20 antibody is available for research use only.
Localization:
Predominantly cell surface with some cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P11836